类器官是在体外由成体干细胞（ASCs）或多能干细胞（PSCs）制备的微型器官。它们通过干细胞的自组织、自我更新和分化能力形成三维（3D）组织样结构。与传统的二维（2D）培养细胞或球体相比，类器官在解剖学上和功能上接近活体器官；由患者肿瘤组织衍生的肿瘤类器官可以保持天然肿瘤组织的遗传异质性，并对个体患者的药物反应具有预测价值。因此类器官越来越多地作为一种有效的模型被广泛应用于药物筛选、药效评估以及发育机制的研究。
类器官培养体系
体外培养类器官的尝试由来已久，但类器官培养一直以来并非易事。从组织样本的获得与保存，类器官培养体系的建立，到类器官的鉴定，类器官的稳定冻存及传代，每一步都可谓是困难重重。根据最新的PubMed检索数据，输入关键词“organoid”，可以看到约20,000+篇可参考文献。即使针对特定的类器官，相关的文章至少也有几十篇。面对如此众多的文章，如此不尽相同的复杂培养体系，如何快速精准地走出第一步即成功构建类器官培养体系显得尤为重要。
近岸蛋白依靠专业的研发团队，精心打造类器官验证平台，可提供一系列经验证、高活性、高稳定性的类器官培养产品，助您高效快速开展类器官实验！
经类器官培养验证的细胞因子
近岸蛋白自主研发生产并经过类器官培养验证的低内毒素Activin A、BMP-4、EGF、FGF-7、FGF-10、FGFb、FGF8b、HGF、IGF1、Noggin、NRG1、R-Spondin 1/3和Wnt3a等细胞因子，已经在人肿瘤类器官如垂体瘤类器官、脑膜瘤类器官、乳腺癌类器官，人正常类器官如ipsc来源的人小肠类器官，以及小鼠正常类器官如小肠类器官、结肠类器官、胃类器官以及胆管类器官等体外模型上进行了验证。
验证数据
乳腺癌类器官
Fluorescent image of human breast cancer organoid structure（ DAPI (blue), Ki67(red) and ER(green) ）.Human breast cancer organoids were cultured with Wnt3a (Cat#C22R), Noggin (Cat#CB89) , R-spondin 3 (Cat#C18C) , KGF(Cat#CH73), FGF-10 (Cat#CR11), EGF (Cat#C029) and NRG1-beta 1 (Cat#C753).
ipsc来源的人小肠类器官
Human intestinal organoids (ipsc dereived) were cultured with Activin A(Cat#C687), BMP-4(Cat#CR93) , EGF (Cat#C029), Wnt3a (Cat#C22R), Noggin (Cat#CB89), R-spondin 1 (Cat#CX83) and FGF-4 (Cat#CR08) . The organoids showed good morphology.
小鼠小肠类器官
Mouse intestinal organoids were cultured with EGF (Cat#C029), Wnt3a (Cat#C22R), Noggin (Cat#C028) and R-spondin 1 (Cat#CX83). After five days of culture, the organoids showed good morphology and germination rate.
小鼠结肠类器官
Mouse Colon organoids were cultured with EGF (Cat#C029), Wnt3a (Cat#C22R), Noggin (Cat#C028) , and R-spondin 1 (Cat#CX83). The organoids showed good morphology.
小鼠胃类器官
Mouse Gastric organoids were cultured with EGF (Cat#C029), Wnt3a (Cat#C22R), Noggin (Cat#C028) , R-spondin 1 (Cat#CX83) and FGF-10 (Cat#CR11). The organoids showed good morphology.
小鼠胆管类器官
Mouse bile duct organoids were cultured with EGF (Cat#C029) , R-spondin 1 (Cat#CX83) ,FGF-10 (Cat#CR11) and HGF (Cat#CJ72). The organoids showed good morphology.
经类器官培养验证的培养基
近岸蛋白提供一系列经验证的类器官培养基，包括肠癌、胃癌、肝癌、肺癌、乳腺癌、胰腺癌、甲状腺癌、食管癌、宫颈癌、肾癌、脑胶质瘤等肿瘤类器官培养基，以及肝、肺等正常组织类器官培养基。
验证数据
人结直肠癌
Human colorectal cancer-derived organoids were cultured with Intestinal Cancer Organoid Culture Medium(Cat#OCMHC01). The organoids showed good morphology.(Data were kindlyprovided by Novoprotein's customer)
人肾癌
Human renal cancer-derived organoids were cultured with Renal Cancer Organoid Culture Medium(Cat#OCMHC02).The organoids showed good morphology. (Data were kindly provided by KOUSAl, Co., Ltd.)
人乳腺癌
Human breast cancer-derived organoids were cultured with Breast Cancer Organoid Culture Medium(Cat#OCMHC06).The organoids showed good morphology. (Data were kindly provided by Novoprotein's customer)
人肺癌
Human lung cancer-derived organoids were cultured with Lung Cancer Organoid Culture Medium(Cat#OCMHC05).The organoids showed good morphology. (Data were kindly provided by Novoprotein's customer)
相关产品：
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类器官培养类型 |
相关因子 |
|
胃类器官 |
Activin A、BMP-4、EGF、FGF-4、FGF-10、Noggin、R-spondin 1、Wnt3a
|
|
肠类器官 |
Activin A、BMP-4、EGF、FGF-4、Noggin、R-spondin 1、Wnt3a |
|
肝类器官 |
Activin A、BMP-4、BMP-7、FGF-7、FGF-10、FGF-19、HGF、OSM、R-Spondin 1
|
|
肺类器官 |
Activin A、FGF-4、FGF-7、FGF-10、Noggin、SHH、Wnt3a |
|
前列腺类器官 |
EGF、FGF-10、Noggin、R-spondin 1、Wnt-3a |
|
脑类器官 |
BDNF、EGF、FGF basic、GDNF、NT-3 |
|
内耳类器官 |
BMP-4、FGF basic |
|
视网膜类器官 |
SHH、Wnt-3a |
|
乳腺类器官 |
EGF、FGF basic、FGF-7、FGF-10、Heregulinβ-1、Noggin、R-Spondin 1、R-Spondin 3、Wnt-3a |
|
肾脏类器官 |
BMP-2、BMP-4、BMP-7、FGF basic、FGF-9、Activin A、FGF-7、GDNF、R-Spondin 1 |
|
心脏类器官 |
Activin A、BMP-4、FGF basic、TGF-beta 1、Transferrin |
|
血管类器官 |
BMP-4、FGF basic、VEGF |
