胰蛋白酶被用于酶促释放贴壁细胞在组织培养平板上进行传代。
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0.25% Trypsin/2.21 mM EDTA in HBSS without sodium bicarbonate, calcium and magnesium Porcine Parvovirus Tested.
Trypsin is used to enzymatically release adherent cells from tissue culture plates for passaging. Divalent cations such as calcium and magnesium, which are often present in the cell culture environment, inhibit this action. EDTA sequesters these ions and thus enhances the efficacy of trypsin. This product contains trypsin at a concentration of 0.25% and 2.21 mM EDTA.