0.25% Trypsin/2.21 mM EDTA in HBSS without sodium bicarbonate, calcium and
magnesium Porcine Parvovirus Tested.
Trypsin is used to enzymatically release adherent cells from tissue culture
plates for passaging. Divalent cations such as calcium and magnesium, which are
often present in the cell culture environment, inhibit this action. EDTA
sequesters these ions and thus enhances the efficacy of trypsin. This product
contains trypsin at a concentration of 0.25% and 2.21 mM EDTA.