现货 Roche Dispase II/分散酶（5*1G）04942078001

Roche Dispase II/分散酶（5*1G）04942078001

Dispase（分散酶）是中性蛋白酶的一种，可用于从广泛组织中分离制备细胞。

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货品编号：

04942078001

品牌：

Roche

数量： -+ 支

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品牌：Roche
所属分类：Dispase

产品特点
Rapid, effective, yet gentle agent that liberates cells with minimal cell 
damage
Maintains cell membrane integrity
Non-mammalian source - free of mycoplasma and animal virus contamination
Extremely stable to influences of temperature, pH, and interference by serum 
components
Easily inactivated by chelating agents or by dilution
Delivers higher activity and convenience
 
内含组分
5 vials with 1 g each; non-sterile lyophilizate
Preparation of Stock and Working Solution:
Dissolve the nonsterile lyophilized enzyme in Hepes-buffered saline (50 mM 
Hepes/KOH pH 7.4, 150 mM NaCl) (10 mg/ml). Dilute further with the culture 
medium to be used for the isolated cells to a final concentration of 0.6 to 2.4 
U/ml. Concentrations higher than 2.4 U/ml are not recommended. Sterilize through 
a 0.22 μm filter membrane.
 
应用
Dispase is used for the preparation of cells from a wide variety of different 
tissues and organs. The enzyme has proven to be a rapid and effective, yet 
gentle agent for the disruption of extracellular matrix of tissues for releasing 
single cells for cell culture (primary cell culture) or harvesting cells already 
in culture to transfer to new substrate (secondary culture). Furthermore, it is 
used to prevent unwanted clumping of cells cultured in suspension.

Roche Dispase II/分散酶（5*1G）04942078001

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