cDNA文库构建服务

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cDNA文库构建服务

cDNA 文库是指某生物某发育时期所转录的全部 mRNA 经反转录形成的 cDNA 片段与某种载体连接而形成的克隆的集合。

cDNA文库构建服务

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所属分类：文库/载体构建

<p><span style="font-size: 14px; line-height: 18px; font-family: Tahoma; color: rgb(38, 38, 38);">文库构建是指利用载体克隆技术形成的克隆集合。通过构建文库我们可以用于分离全长基因进而开展基因功能研究；从文库中筛选到所需的目的基因，并直接用于该目的基因的表达；在研究具体某类特定细胞中基因组的表达状态及表达基因的功能鉴定方便具有特殊的优势。我们可提供全方位的文库构建选择，并对库容量、插入片段大小和重组率进行严格</span><font style="padding: 0px; margin: 0px; font-size: 14px; line-height: 18px; font-family: Tahoma; color: rgb(38, 38, 38); word-break: break-all;" face="Tahoma">QC</font><font style="padding: 0px; line-height: 18px; margin: 0px; color: rgb(38, 38, 38); font-size: 14px; word-break: break-all;" face="微软雅黑">。</font></p>
<font face="微软雅黑" color="#262626">
<p style="padding: 0px; margin: 0pt 0px; font-size: 14px; line-height: 21px; font-family: 宋体, Verdana, Arial, Helvetica, sans-serif; word-break: break-all;"><span style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.5; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; FONT-FAMILY: Tahoma; FONT-SIZE: 10.5pt; WORD-BREAK: break-all; PADDING-TOP: 0px"><strong>服务类别</strong></span></p>
<p style="padding: 0px; margin: 0pt 0px; font-size: 14px; line-height: 21px; font-family: 宋体, Verdana, Arial, Helvetica, sans-serif; word-break: break-all;"><span style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.5; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; FONT-FAMILY: Tahoma; FONT-SIZE: 10.5pt; WORD-BREAK: break-all; PADDING-TOP: 0px"><strong>Smart<font style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.3em !important; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; WORD-BREAK: break-all; PADDING-TOP: 0px" face="微软雅黑">全长</font><font style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.3em !important; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; WORD-BREAK: break-all; PADDING-TOP: 0px" face="Tahoma">cDNA</font></strong><font style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.3em !important; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; WORD-BREAK: break-all; PADDING-TOP: 0px" face="微软雅黑"><strong>文库构建：</strong>采用</font><font style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.3em !important; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; WORD-BREAK: break-all; PADDING-TOP: 0px" face="Tahoma">Clonetech SMART</font><font style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.3em !important; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; WORD-BREAK: break-all; PADDING-TOP: 0px" face="微软雅黑">专利技术和降低</font><font style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.3em !important; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; WORD-BREAK: break-all; PADDING-TOP: 0px" face="Tahoma">RNA</font><font style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.3em !important; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; WORD-BREAK: break-all; PADDING-TOP: 0px" face="微软雅黑">酶</font><font style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.3em !important; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; WORD-BREAK: break-all; PADDING-TOP: 0px" face="Tahoma">H</font><font style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.3em !important; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; WORD-BREAK: break-all; PADDING-TOP: 0px" face="微软雅黑">活性的反转录酶，有效保障文库的代表性</font></span></p>
<p style="padding: 0px; margin: 0pt 0px; font-size: 14px; line-height: 21px; font-family: 宋体, Verdana, Arial, Helvetica, sans-serif; word-break: break-all;"><span style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.5; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; FONT-FAMILY: Tahoma; FONT-SIZE: 10.5pt; WORD-BREAK: break-all; PADDING-TOP: 0px"><strong>酵母双杂交<font style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.3em !important; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; WORD-BREAK: break-all; PADDING-TOP: 0px" face="Tahoma">cDNA</font></strong><font style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.3em !important; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; WORD-BREAK: break-all; PADDING-TOP: 0px" face="微软雅黑"><strong>文库构建：</strong>采用</font><font style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.3em !important; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; WORD-BREAK: break-all; PADDING-TOP: 0px" face="Tahoma">Clontech</font><font style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.3em !important; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; WORD-BREAK: break-all; PADDING-TOP: 0px" face="微软雅黑">的酵母双杂交载体，可用于检测存在于蛋白质之间的微弱的或暂时的相互作用</font></span></p>
<p style="padding: 0px; margin: 0pt 0px; font-size: 14px; line-height: 21px; font-family: 宋体, Verdana, Arial, Helvetica, sans-serif; word-break: break-all;"><span style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.5; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; FONT-FAMILY: Tahoma; FONT-SIZE: 10.5pt; WORD-BREAK: break-all; PADDING-TOP: 0px"><strong>高级全长<font style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.3em !important; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; WORD-BREAK: break-all; PADDING-TOP: 0px" face="Tahoma">cDNA</font></strong><font style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.3em !important; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; WORD-BREAK: break-all; PADDING-TOP: 0px" face="微软雅黑"><strong>文库构建：</strong>利用独特的富集含</font><font style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.3em !important; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; WORD-BREAK: break-all; PADDING-TOP: 0px" face="Tahoma">5'</font><font style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.3em !important; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; WORD-BREAK: break-all; PADDING-TOP: 0px" face="微软雅黑">帽子结构的全长</font><font style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.3em !important; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; WORD-BREAK: break-all; PADDING-TOP: 0px" face="Tahoma">cDNA</font><font style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.3em !important; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; WORD-BREAK: break-all; PADDING-TOP: 0px" face="微软雅黑">技术，确保获得高度富集的全长</font><font style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.3em !important; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; WORD-BREAK: break-all; PADDING-TOP: 0px" face="Tahoma">cDNA</font><font style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.3em !important; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; WORD-BREAK: break-all; PADDING-TOP: 0px" face="微软雅黑">文库</font></span></p>
<p style="padding: 0px; margin: 0pt 0px; font-size: 14px; line-height: 21px; font-family: 宋体, Verdana, Arial, Helvetica, sans-serif; word-break: break-all;"><span style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.5; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; FONT-FAMILY: Tahoma; FONT-SIZE: 10.5pt; WORD-BREAK: break-all; PADDING-TOP: 0px"><strong>均一化<font style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.3em !important; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; WORD-BREAK: break-all; PADDING-TOP: 0px" face="Tahoma">cDNA</font></strong><font style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.3em !important; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; WORD-BREAK: break-all; PADDING-TOP: 0px" face="微软雅黑"><strong>文库构建：</strong>基于双链特异性核酸酶（</font><font style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.3em !important; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; WORD-BREAK: break-all; PADDING-TOP: 0px" face="Tahoma">DSN</font><font style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.3em !important; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; WORD-BREAK: break-all; PADDING-TOP: 0px" face="微软雅黑">）的先进</font><font style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.3em !important; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; WORD-BREAK: break-all; PADDING-TOP: 0px" face="Tahoma">cDNA</font><font style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.3em !important; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; WORD-BREAK: break-all; PADDING-TOP: 0px" face="微软雅黑">文库均一化技术，将</font><font style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.3em !important; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; WORD-BREAK: break-all; PADDING-TOP: 0px" face="Tahoma">cDNA</font><font style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.3em !important; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; WORD-BREAK: break-all; PADDING-TOP: 0px" face="微软雅黑">文库中高丰度的序列拷贝数减少</font><font style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.3em !important; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; WORD-BREAK: break-all; PADDING-TOP: 0px" face="Tahoma">100</font><font style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.3em !important; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; WORD-BREAK: break-all; PADDING-TOP: 0px" face="微软雅黑">－</font><font style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.3em !important; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; WORD-BREAK: break-all; PADDING-TOP: 0px" face="Tahoma">200</font><font style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.3em !important; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; WORD-BREAK: break-all; PADDING-TOP: 0px" face="微软雅黑">倍，增加克隆低丰度</font><font style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.3em !important; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; WORD-BREAK: break-all; PADDING-TOP: 0px" face="Tahoma">mRNA</font><font style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.3em !important; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; WORD-BREAK: break-all; PADDING-TOP: 0px" face="微软雅黑">的机会</font></span></p>
<p style="padding: 0px; margin: 0pt 0px; font-size: 14px; line-height: 21px; font-family: 宋体, Verdana, Arial, Helvetica, sans-serif; word-break: break-all;"><span style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.5; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; FONT-FAMILY: Tahoma; FONT-SIZE: 10.5pt; WORD-BREAK: break-all; PADDING-TOP: 0px"><strong>消减<font style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.3em !important; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; WORD-BREAK: break-all; PADDING-TOP: 0px" face="Tahoma">cDNA</font></strong><font style="PADDING-BOTTOM: 0px; LINE-HEIGHT: 1.3em !important; MARGIN: 0px; PADDING-LEFT: 0px; PADDING-RIGHT: 0px; WORD-BREAK: break-all; PADDING-TOP: 0px" face="微软雅黑"><strong>文库构建：</strong>分离两种细胞或两种组织的细胞中的差异表达基因</font></span></p>
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